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Following this, unilateral L5 spinal nerve ligation was performed. Seven times afterwards, sensory thresholds were being reassessed and the development of allodynia to chilly and mechanical stimuli and hyperalgesia to lionwin55 a noxious thermal stimulus confirmed.
The hind paw withdrawal latency (s) to this stimulus was tested three times at intervals of not lower than 3 min as well as a suggest calculated. The system has an computerized Minimize-off at 21 s to stop the potential risk of thermal injuries towards the skin.
the CB1 receptor. Administration of SR141716a on your own experienced no affect around the noticed allodynia and hyperalgesia, which won't assistance the strategy of an endogenous analgesic tone.
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